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biotinylated wisteria floribunda lectin (wfa, wfl)  (Vector Laboratories)


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    Structured Review

    Vector Laboratories biotinylated wisteria floribunda lectin (wfa, wfl)
    Biotinylated Wisteria Floribunda Lectin (Wfa, Wfl), supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 351 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/B-1355-2/Biotinylated+Wisteria+Floribunda+Lectin+(WFA%2C+WFL)/custom%40b-1355%4042557328
    Average 96 stars, based on 351 article reviews
    biotinylated wisteria floribunda lectin (wfa, wfl) - by Bioz Stars, 2026-09
    96/100 stars

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    Incubation:

    Article Title: Mechanical properties of the developing brain in a model of fetal alcohol spectrum disorders and relationships to perineuronal net integrity.
    Article Snippet: Neuroimaging is a useful tool for examining altered neurodevelopmental trajectories in fetal alcohol spectrum disorders (FASD).. FASD affects 1 in 20 infants in the United States with higher prevalence in specific regions across the globe.. Advanced neuroimaging methods, such as volumetric morphometry and diffusion-weighted imaging, are critical for determining the effectiveness of interventions that support neurodevelopment in FASD.

    Article Title: Altered lipid peroxidation, perineuronal net and oligodendrocyte markers in the frontal cortex of a dual-hit neurodevelopmental model support its relevance to schizophrenia
    Article Snippet: For 3,3′-diaminobenzidine (DAB) labeling of PNNs sections were incubated (30 min) in a solution of 1.5% H 2 O 2 (Sigma-Aldrich), 10% methanol (Honeywell) and 0.4% Triton-X100 (Sigma-Aldrich) in PBS, to block endogenous peroxidase activity, then washed (2 × 5 min) with PBS. .. They were incubated (1 h) in 5% normal horse serum (Vector Laboratories) and 0.4% Triton-X100 in PBS to minimize non-specific binding, followed by (overnight, 4 °C) Wisteria floribunda agglutinin (WFA; Vector Laboratories, B-1355-2, 1:1000 in blocking solution) which is a lectin that binds chondroitin sulfate chains in PNNs. .. Sections were washed (3 × 5 min) in PBS, incubated (45 min) in Vectastain ® avidin-biotin complex (ABC solution that had been pre-mixed for 30 min; Vector Laboratories, PK-4000), washed (2 × 5 min) in PBS then incubated (45 s) in DAB solution with nickel (Vector Laboratories, SK-4100).

    Binding Assay:

    Article Title: Altered lipid peroxidation, perineuronal net and oligodendrocyte markers in the frontal cortex of a dual-hit neurodevelopmental model support its relevance to schizophrenia
    Article Snippet: For 3,3′-diaminobenzidine (DAB) labeling of PNNs sections were incubated (30 min) in a solution of 1.5% H 2 O 2 (Sigma-Aldrich), 10% methanol (Honeywell) and 0.4% Triton-X100 (Sigma-Aldrich) in PBS, to block endogenous peroxidase activity, then washed (2 × 5 min) with PBS. .. They were incubated (1 h) in 5% normal horse serum (Vector Laboratories) and 0.4% Triton-X100 in PBS to minimize non-specific binding, followed by (overnight, 4 °C) Wisteria floribunda agglutinin (WFA; Vector Laboratories, B-1355-2, 1:1000 in blocking solution) which is a lectin that binds chondroitin sulfate chains in PNNs. .. Sections were washed (3 × 5 min) in PBS, incubated (45 min) in Vectastain ® avidin-biotin complex (ABC solution that had been pre-mixed for 30 min; Vector Laboratories, PK-4000), washed (2 × 5 min) in PBS then incubated (45 s) in DAB solution with nickel (Vector Laboratories, SK-4100).

    Blocking Assay:

    Article Title: Altered lipid peroxidation, perineuronal net and oligodendrocyte markers in the frontal cortex of a dual-hit neurodevelopmental model support its relevance to schizophrenia
    Article Snippet: For 3,3′-diaminobenzidine (DAB) labeling of PNNs sections were incubated (30 min) in a solution of 1.5% H 2 O 2 (Sigma-Aldrich), 10% methanol (Honeywell) and 0.4% Triton-X100 (Sigma-Aldrich) in PBS, to block endogenous peroxidase activity, then washed (2 × 5 min) with PBS. .. They were incubated (1 h) in 5% normal horse serum (Vector Laboratories) and 0.4% Triton-X100 in PBS to minimize non-specific binding, followed by (overnight, 4 °C) Wisteria floribunda agglutinin (WFA; Vector Laboratories, B-1355-2, 1:1000 in blocking solution) which is a lectin that binds chondroitin sulfate chains in PNNs. .. Sections were washed (3 × 5 min) in PBS, incubated (45 min) in Vectastain ® avidin-biotin complex (ABC solution that had been pre-mixed for 30 min; Vector Laboratories, PK-4000), washed (2 × 5 min) in PBS then incubated (45 s) in DAB solution with nickel (Vector Laboratories, SK-4100).



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    Vector Laboratories wisteria floribunda lectin wfa
    B4GALNT4 stabilizes PDK1 through post‐translational glycosylation. (A) IHC staining images of B4GALNT4 and <t>WFA</t> staining for GalNAc‐containing glycans in clinical PCa tissues, classified into strong and weak expression groups based on B4GALNT4 protein intensity (scale bar: 250 µm). The right‐hand bar graph illustrates that higher B4GALNT4 protein levels are associated with increased WFA staining intensity. (B, C) <t>Lectin</t> blot analysis with biotinylated WFA of lysates from C4‐2 (B) or LNCaP (C) cells transfected with control (shNC) or B4GALNT4‐targeting shRNAs (sh1 # , sh2 # ). Silver staining of the same gel is shown as a loading control. (D, E) WFA immunofluorescence staining (green) in C4‐2 (D) and LNCaP (E) cells after B4GALNT4 knockdown, confirming the reduction in cell‐associated GalNAc‐containing glycans. Nuclei are counterstained with DAPI (blue) (scale bar: 50 µm). (F, G) Western blotting (F) and qRT‐PCR (G) of B4GALNT4 and PDK1 expression in 293T cells transfected with Flag‐B4GALNT4 or HA‐PDK1, respectively. Overexpression of Flag‐B4GALNT4 in 293T cells resulted in the detection of a full‐length B4GALNT4 band at approximately 116 kDa (F). Endogenous B4GALNT4 was predominantly detected at approximately 85 kDa. (H‐I) Western blotting (H) and qRT‐PCR (I) of B4GALNT4 and PDK1 expression in B4GALNT4‐knockdown C4‐2 or LNCaP cells. (J, K) Western blotting of PDK1 and B4GALNT4 in C4‐2 (J) and LNCaP (K) cells treated with CHX. Quantification of PDK1 expression is shown on the right. (L, M) Western blotting of PDK1 and B4GALNT4 in B4GALNT4‐knockdown C4‐2 and LNCaP cells treated with MG132 (L) or CQ (M). Experiments were conducted in triplicate, and data are presented as mean ± SD ( * , p < 0.05; ** , p < 0.01; *** , p < 0.001; NS, p > 0.05). sh1 # , shB4GALNT4 1# ; sh2 # , shB4GALNT4 2# .
    Wisteria Floribunda Lectin Wfa, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/B-1355-2/Biotinylated+Wisteria+Floribunda+Lectin+(WFA%2C+WFL)/pmc13104079-192-23-28
    Average 96 stars, based on 1 article reviews
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    Image Search Results


    B4GALNT4 stabilizes PDK1 through post‐translational glycosylation. (A) IHC staining images of B4GALNT4 and WFA staining for GalNAc‐containing glycans in clinical PCa tissues, classified into strong and weak expression groups based on B4GALNT4 protein intensity (scale bar: 250 µm). The right‐hand bar graph illustrates that higher B4GALNT4 protein levels are associated with increased WFA staining intensity. (B, C) Lectin blot analysis with biotinylated WFA of lysates from C4‐2 (B) or LNCaP (C) cells transfected with control (shNC) or B4GALNT4‐targeting shRNAs (sh1 # , sh2 # ). Silver staining of the same gel is shown as a loading control. (D, E) WFA immunofluorescence staining (green) in C4‐2 (D) and LNCaP (E) cells after B4GALNT4 knockdown, confirming the reduction in cell‐associated GalNAc‐containing glycans. Nuclei are counterstained with DAPI (blue) (scale bar: 50 µm). (F, G) Western blotting (F) and qRT‐PCR (G) of B4GALNT4 and PDK1 expression in 293T cells transfected with Flag‐B4GALNT4 or HA‐PDK1, respectively. Overexpression of Flag‐B4GALNT4 in 293T cells resulted in the detection of a full‐length B4GALNT4 band at approximately 116 kDa (F). Endogenous B4GALNT4 was predominantly detected at approximately 85 kDa. (H‐I) Western blotting (H) and qRT‐PCR (I) of B4GALNT4 and PDK1 expression in B4GALNT4‐knockdown C4‐2 or LNCaP cells. (J, K) Western blotting of PDK1 and B4GALNT4 in C4‐2 (J) and LNCaP (K) cells treated with CHX. Quantification of PDK1 expression is shown on the right. (L, M) Western blotting of PDK1 and B4GALNT4 in B4GALNT4‐knockdown C4‐2 and LNCaP cells treated with MG132 (L) or CQ (M). Experiments were conducted in triplicate, and data are presented as mean ± SD ( * , p < 0.05; ** , p < 0.01; *** , p < 0.001; NS, p > 0.05). sh1 # , shB4GALNT4 1# ; sh2 # , shB4GALNT4 2# .

    Journal: Advanced Science

    Article Title: B4GALNT4‐Mediated Glycosylation of PDK1 Activates the PI3K‐AKT Signaling Pathway to Promote Prostate Cancer Progression

    doi: 10.1002/advs.202511293

    Figure Lengend Snippet: B4GALNT4 stabilizes PDK1 through post‐translational glycosylation. (A) IHC staining images of B4GALNT4 and WFA staining for GalNAc‐containing glycans in clinical PCa tissues, classified into strong and weak expression groups based on B4GALNT4 protein intensity (scale bar: 250 µm). The right‐hand bar graph illustrates that higher B4GALNT4 protein levels are associated with increased WFA staining intensity. (B, C) Lectin blot analysis with biotinylated WFA of lysates from C4‐2 (B) or LNCaP (C) cells transfected with control (shNC) or B4GALNT4‐targeting shRNAs (sh1 # , sh2 # ). Silver staining of the same gel is shown as a loading control. (D, E) WFA immunofluorescence staining (green) in C4‐2 (D) and LNCaP (E) cells after B4GALNT4 knockdown, confirming the reduction in cell‐associated GalNAc‐containing glycans. Nuclei are counterstained with DAPI (blue) (scale bar: 50 µm). (F, G) Western blotting (F) and qRT‐PCR (G) of B4GALNT4 and PDK1 expression in 293T cells transfected with Flag‐B4GALNT4 or HA‐PDK1, respectively. Overexpression of Flag‐B4GALNT4 in 293T cells resulted in the detection of a full‐length B4GALNT4 band at approximately 116 kDa (F). Endogenous B4GALNT4 was predominantly detected at approximately 85 kDa. (H‐I) Western blotting (H) and qRT‐PCR (I) of B4GALNT4 and PDK1 expression in B4GALNT4‐knockdown C4‐2 or LNCaP cells. (J, K) Western blotting of PDK1 and B4GALNT4 in C4‐2 (J) and LNCaP (K) cells treated with CHX. Quantification of PDK1 expression is shown on the right. (L, M) Western blotting of PDK1 and B4GALNT4 in B4GALNT4‐knockdown C4‐2 and LNCaP cells treated with MG132 (L) or CQ (M). Experiments were conducted in triplicate, and data are presented as mean ± SD ( * , p < 0.05; ** , p < 0.01; *** , p < 0.001; NS, p > 0.05). sh1 # , shB4GALNT4 1# ; sh2 # , shB4GALNT4 2# .

    Article Snippet: After blocking with goat serum (AR0009; BOSTER), slides were incubated overnight at 4°C with primary antibodies: B4GALNT4 (orb546266; Biorbyt), PDK1 (sc‐17765; Santa Cruz), Wisteria Floribunda Lectin (WFA) (B‐1355‐2; Vector Laboratories), phosphorylated AKT (p‐AKT) (sc‐514032; Santa Cruz), and Ki‐67 (A20018; Abclonal).

    Techniques: Glycoproteomics, Immunohistochemistry, Staining, Expressing, Transfection, Control, Silver Staining, Immunofluorescence, Knockdown, Western Blot, Quantitative RT-PCR, Over Expression